FIELD: chemistry, organic, biotechnologies.
SUBSTANCE: invention relates to biotechnology. Comma bacillus cellular mass will be obtained, the proteins will be removed from the surface with orbital rotation of the bacterial mass with the velocity of 2500 r.p.m. during 2 minutes in the vibration mixer. The cellular mass will be fractioned with low velocity centrifuging at 3-4 thousand r.p.m. The supernatant will be fractioned with high velocity centrifuging at 18-20 thousand r.p.m. Then the sediment will be selectively extracted for obtaining supernatant containing purified proteins TCAP and OmpU with the solution 125 mM of ethanolamine (pH 10.5) at 4°C in the shaker. From the supernatant, proteins of toxin-coregulated pili adhesine and OmpU will be isolated.
EFFECT: isolation of proteins of toxin-coregulated pili adhesine and OmpU from supernatant.
3 cl, 3 dwg, 4 ex
