FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology and genetic engineering. Disclosed is a method of producing E. coli BL21 Star™(DE3) pET302/NT-His tcpA – producer of recombinant protein TcpA cholera vibrio biovar El Tor. Method provides for transformation of E. coli BL21 Star™(DE3) strain cells plasmid pET302/NT-His containing a cloned tcpA gene fragment with size of 534 base pairs from plasmid pCR2.1. tcpA, obtained by PCR using oligonucleotide primers F ccgctcgaggattcgcagaatatgactaaggctgc and R gctggatccttaactgttaccaaaagctactgtgaat.
EFFECT: invention provides high-yield TcAA protein.
1 cl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PROTEIN FOR CHOLERA IMMUNIZATION | 2019 |
|
RU2723705C1 |
| STRAIN OF ESCHERICHIA COLI BACTERIA - PRODUCER OF IL-29 RECOMBINANT PROTEIN | 2020 |
|
RU2760585C1 |
| RECOMBINANT PLASMID EXPRESSING CLONED CHAPERONE HFQ VIBRIO CHOLERAE GENE, AND ESCHERICHIA COLI STRAIN - CHAPERONE SUPERFOOD HFQ VIBRIO CHOLERAE | 2018 |
|
RU2707525C1 |
| RECOMBINANT PLASMID EXPRESSING VIBRIO CHOLERAE CLONED CEF GENE (VARIANTS) AND ESCHERICHIA COLI STRAIN-PRODUCER OF VIBRIO CHOLERAE CEF (VARIANTS) | 2005 |
|
RU2313577C2 |
| RECOMBINANT PLASMID EXPRESSING VIBRIO CHOLERAE CLONED Ace GENE AND ESCHERICHIA COLI STRAIN - PRODUCER OF VIBRIO CHOLERAE ACCESSORY CHOLERA ENTEROTOXIN | 2006 |
|
RU2313576C1 |
| RECOMBINANT PLASMID EXPRESSING CLONED GENE OF Vibrio cholerae HEMAGGLUTININ/PROTEASE AND Escherichia coli STRAIN AS PRODUCER OF Vibrio cholerae HEMAGGLUTININ/PROTEASE | 2005 |
|
RU2306337C2 |
| COMPLEX GENE- AND IMMUNODIAGNOSTIC TEST-SYSTEM FOR IDENTIFICATION OF CHOLERA VIBRIONS OF O1 AND O139 SEROGROUPS AND ESTIMATION OF THEIR VIRULENCE | 2009 |
|
RU2404257C1 |
| STRAIN OF BACTERIUM VIBRIO CHOLERAE ELTOR KM-195 AS PRODUCER OF CHOLERAIC TOXIN OF TYPE II, METHOD OF STRAIN PREPARING | 2000 |
|
RU2172776C1 |
| METHOD OF PRODUCING CHOLERA TOXIN FOR CONTROL OF PRODUCTION OF CHOLERA CHEMICAL VACCINE | 2022 |
|
RU2799574C1 |
| DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36, DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG, PLASMID EXPRESSION VECTOR (VERSIONS), ESCHERICHIA COLI BL21 [DE3] STAR (PET-IL36RAF),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 AND STRAIN OF BACTERIA ESCHERICHIA COLI BL21 [DE3] STAR (PET-IL36RAF-HIS),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG | 2014 |
|
RU2582569C2 |
