PRODUCTION METHOD OF ANIMAL COLIBACILLOSIS VACCINE Russian patent published in 2008 - IPC A61K39/02 A61K39/108 C12N1/00 

FIELD: veterinary.

SUBSTANCE: escherichia coli cells containing adhesive gene K 88 ab, K 88 ac, K 88 ad, K 99, F-41, Att-25, 987P are cultivated in liquid nutrient medium to produce vaccine. Physical, chemical and biological indicators are investigated in the process of bacterial bulk growth. In addition, activity of fimbrial adhesin in passive hemagglutination of cellular bacterial bulk is determined. Further cultivation is terminated when fimbrial adhesins titers values are K 88 ab, K 88 ac, K 88 ad, K99, F-41, Att-25, 987P equal to: 1:2048-4096; 1:1024-2048; 1:256-512; 1:1024-2056; 1:1024-2056; 1:32-64; 1:256-512, accordingly. Biological bulk is then concentrated and spinned at 3000-5000 rev/min for 20-25 minutes to separate culture broth from cellular bacterial bulk. Then vaccine is inactivate.

EFFECT: improvement of target product quality.

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