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2 325 438

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IPC

C12N15/17(2006-01-01)

C12N1/21(2006-01-01)

C12R1/19(2006-01-01)

(21) (22)

Application

2006140992/13, 2006-11-21

(24)

Start date

2006-11-21

(22)

Actual filing date

2006-11-21

(45)

Published

2008-05-27

(72)

Inventor

Patrushev Lev IvanovichZinchenko Aleksej AlekseevichGordeeva Elena Anatol'EvnaKostromina Tat'Jana IvanovnaPatrusheva Natal'Ja L'VovnaBairamashvili Dmitrij Il'IchMiroshnikov Anatolij Ivanovich

(73)

Holder

Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Rossijskoj Akademii Nauk

STRAIN OF BACTERIA ESCHERICHIA COLI BLP21-PRODUCER OF RECOMBINANT HUMAN PROINSULIN LYSPRO Russian patent published in 2008 - IPC C12N15/17 C12N1/21 C12R1/19

Abstract RU 2325438 C1

FIELD: organic chemistry, genetics.

SUBSTANCE: invention refers to genetic engineering and can be applied for production of high-purity engineered insulin Lyspro that is immediate-action analogue of human insulin. Transformation of parent strain of bacteria Escherichia coli BL21 of plasmid DNA pLP-3-1 results in strain Escherichia coli BLP21-producer of proinsulin Lyspro. The invention enables to produce stable high yield recombinant insulin Lyspro and simplify production technology.

EFFECT: production of stable high yield recombinant insulin Lyspro and simplify production technology.

1 ex, 1 dwg

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RU 2 325 438 C1

Authors

Patrushev Lev Ivanovich

Zinchenko Aleksej Alekseevich

Gordeeva Elena Anatol'Evna

Kostromina Tat'Jana Ivanovna

Patrusheva Natal'Ja L'Vovna

Bairamashvili Dmitrij Il'Ich

Miroshnikov Anatolij Ivanovich

Dates

2008-05-27—Published

2006-11-21—Filed