FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of therapeutic proteins, and can be used to produce recombinant proinsulin lisprum human. Recombinant proinsulin is obtained in a hybrid polypeptide, in which the human HGS protein fragment sequence, substrate of tyrosine kinase is connected through peptide linker with sequence GlyHis6GlySerArg with amino acid sequence of proinsulin of lisprum human, with arginine as site of proteolysis between C-peptide and chain A. For this purpose recombinant plasmid DNA pF267 and strain-producer Escherichia coli BL21/pF267 are used.
EFFECT: invention provides synthesis of a hybrid polypeptide with an expression level of not less than 4_5 % of the crude weight of the cell biomass, enables increasing the proportion of insulin in the hybrid protein to 49_5 % and improving the purification efficiency of the end product by eliminating the closely related Arg0-A-lyspro admixture.
3 cl, 5 ex, 7 dwg
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PF265 CODING HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN, AND BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2728611C1 |
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2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
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- Latypov Vitaliy Felixovich
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|
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2019 |
- Latypov Vitalii Feliksovich
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|
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1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Vul'Fson A.N.
- Kostromina T.I.
- Miroshnikov A.I.
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- Kalinin Ju.T.
- Stepanov A.V.
|
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1999 |
- Korobko V.G.
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2004 |
- Shmatchenko V.V.
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|
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2013 |
- Shmatchenko Vadim Vasil'Evich
|
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2013 |
- Shmatchenko Vadim Vasil'Evich
|
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