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2 337 964

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IPC

C12N15/17(2006-01-01)

C12N15/70(2006-01-01)

C12N1/21(2006-01-01)

C12R1/19(2006-01-01)

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Application

2006140999/13, 2006-11-21

(24)

Start date

2006-11-21

(22)

Actual filing date

2006-11-21

(45)

Published

2008-11-10

(72)

Inventor

Patrushev Lev IvanovichZinchenko Aleksej AlekseevichKostromina Tat'Jana IvanovnaMelikhova Tat'Jana DmitrievnaPatrusheva Natal'Ja L'VovnaBairamashvili Dmitrij Il'IchMiroshnikov Anatolij Ivanovich

(73)

Holder

Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Rossijskoj Akademii Nauk

RECOMBINANT PLASMID DNA pAS-2 ENCODING HUMAN BEING PROINSULIN POLYPEPTIDE Aspart AND STRAIN OF BACTERIA Escherichia coli BAS2-RECOMBINANT PROINSULIN Aspart PRODUCER Russian patent published in 2008 - IPC C12N15/17 C12N15/70 C12N1/21 C12R1/19

Abstract RU 2337964 C2

FIELD: chemistry, pharmacology.

SUBSTANCE: plasmid pAS-2 is formed by HindIII/BamHI-fragment of plasmid pPINS07 incorporating a part of artificial gene encoding lgG-binding protein domain A from S. aureus and peptide His₆GlySerArg, and HindIII/BamHI-fragment encoding proinsulin Aspart. Plasmid pAS-2 ensures in cells E. coli the biosynthesis of hybrid protein wherein B domain lineage of staphylococcal protein A Staphylococcus aureus is linked via peptide linker His₆GlySerArg with amino acid sequence of human being proinsulin Aspart. The human being strain of bacteria Escherichia coli BAS2 produced by transforming the cells of the strain by plasmid pAS-2 produces the said hybrid protein.

EFFECT: production of human being insulin Aspart by simplified process with high yield.

2 cl, 4 dwg, 4 ex

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RU 2 337 964 C2

Authors

Patrushev Lev Ivanovich

Zinchenko Aleksej Alekseevich

Kostromina Tat'Jana Ivanovna

Melikhova Tat'Jana Dmitrievna

Patrusheva Natal'Ja L'Vovna

Bairamashvili Dmitrij Il'Ich

Miroshnikov Anatolij Ivanovich

Dates

2008-11-10—Published

2006-11-21—Filed