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(19)

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2 700 751

(13)

(51)

IPC

C07K1/16(2006-01-01)

C07K1/18(2006-01-01)

C07K1/20(2006-01-01)

C07K4/04(2006-01-01)

C07K14/52(2006-01-01)

(21) (22)

Application

2018113580, 2018-04-13

(24)

Start date

2018-04-13

(22)

Actual filing date

2018-04-13

(45)

Published

2019-09-19

(72)

Inventor

Kolobov Aleksej AleksandrovichProtasov Evgenij AleksandrovichPolotskij Aleksandr EvgenevichAntipova Tatyana OlegovnaSineva Svetlana Adamovna

(73)

Holder

Federalnoe Gosudarstvennoe Unitarnoe Predpriyatie Nauchno-Issledovatelskij Institut Osobo Chistykh Biopreparatov" Federalnogo Mediko-Biologicheskogo Agentstva

METHOD FOR PURIFICATION OF RECOMBINANT IL-36RA (EMBODIMENTS) Russian patent published in 2019 - IPC C07K1/16 C07K1/18 C07K1/20 C07K4/04 C07K14/52

Abstract RU 2700751 C1

FIELD: biotechnologies.

SUBSTANCE: invention relates to the biotechnology. Disclosed is a method of purifying the recombinant receptor antagonist interleukin-36. Method of purifying the recombinant receptor antagonist IL-36 involves successively carrying out lysis of cells of the producer strain, ion-exchange chromatography and hydrophobic chromatography. In one embodiment of the method, negative anion-exchange chromatography is used as an ion-exchange chromatography, in another embodiment, cation-exchange chromatography.

EFFECT: invention allows to produce IL-36RA preparation with protein output of 34–57 % of initial amount of protein in biomass of producer strain, purity of not less than 95 % of total amount of protein by RP-HPLC and fraction of deamidated form of protein less than 5 % of total amount of protein.

3 cl, 13 dwg, 3 tbl, 15 ex

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RU 2 700 751 C1

Authors

Kolobov Aleksej Aleksandrovich

Protasov Evgenij Aleksandrovich

Polotskij Aleksandr Evgenevich

Antipova Tatyana Olegovna

Sineva Svetlana Adamovna

Dates

2019-09-19—Published

2018-04-13—Filed