FIELD: biotechnologies.
SUBSTANCE: method of obtaining a continuous cell line capable of maintaining growth of cell-associated alpha herpes viruses involves the infection or transfection of a cell with the nucleic acid or fragment of the herpes virus, the selection of cells expressing the said nucleic acid or its fragment, and the cultivation of the said infected or transfected cell or its progeny in conditions suitable for the expression of the said nucleic acid and the multiplication of the said cell or its progeny. The said nucleic acid includes the herpes virus glycoprotein gene gE or its functional fragment. The expression of the said gE gene or its functional fragment takes place in a sustainable manner. The cell of the continuous cell line capable of maintaining growth of cell-associated alpha herpes viruses includes the herpes virus nucleic acid or its fragment. The said nucleic acid includes the herpes virus glycoprotein gene gE or its functional fragment, the expression of the said gE gene or its functional fragment takes place in a sustainable manner. The said cell can go through at least 10 passages. The invention group includes the application of the said cell for obtaining a vaccine capable of inducing protection against disease in vertebrates, and for obtaining and/or maintaining and/or isolation of a hypervirulent and/or very virulent Marek's disease virus strain, as well as for obtaining the Marek's disease diagnostic antigen. The invention group also includes a method of obtaining and/or isolation and/or maintaining the Marek's disease virus strain. The method involves the infection of the above cell with the said strain and the cultivation of the said cell in conditions suitable for the multiplication of the said cell and obtaining, maintaining, and isolation of the Marek's disease virus.
EFFECT: invention group provides a method of obtaining a vaccine capable of inducing protection against the disease; allows to satisfy the need in new cell systems allowing to maintain new properties acquired by the cell.
35 cl, 6 dwg, 6 ex.
