RECOMBINANT NON-PATHOGENIC STRUCTURES OF MAREK'S DISEASE VIRUS, ENCODING ANTIGENS OF INFECTIOUS LARYNGOTRACHEITIS VIRUS AND INFECTIOUS BURSITIS VIRUS Russian patent published in 2022 - IPC A61K39/12 C12N15/86 A61K39/245 

FIELD: biotechnology.

SUBSTANCE: recombinant herpesvirus of turkey (hereinafter – rHVT) is disclosed for contributing to the protection of poultry from diseases caused by ILTV, IBDV and MDV1. The virus contains the first heterologous nucleic acid and the second heterologous nucleic acid. Both the first heterologous nucleic acid and the second heterologous nucleic acid are located in the same non-essential site. The non-essential site is selected from a group consisting of US2 site and UL54.5 site. The first heterologous nucleic acid contains both a nucleotide sequence, which encodes glycoprotein D of the infectious laryngotracheitis virus (hereinafter – gD ILTV) and a nucleotide sequence, which encodes glycoprotein I of the infectious laryngotracheitis virus (hereinafter – gI ILTV). The second heterologous nucleic acid contains a nucleotide sequence, which encodes viral protein 2 of the infectious bursitis virus (hereinafter – VP2 IBDV). An immunogenic composition for the protection of poultry from a disease caused by ILTV, IBDV and MDV1, containing rHVT, and a vaccine for the protection of poultry from a disease caused by ILTV, IBDV and V1, containing an immunogenic composition, are presented, as well as the use of an immunogenic composition in the production of a vaccine for contributing to the protection of chickens against ILTV and IBDV.

EFFECT: recombinant herpesvirus of turkey is genetically and phenotypically stable with relatively strong antigen expression and efficiency criteria.

11 cl, 2 dwg, 6 tbl, 6 ex