QUANTITATIVE METHOD OF DETERMINING THYMOL AND CARVACROL IN MEDICINAL PLANT RAW MATERIAL Russian patent published in 2008 - IPC G01N33/15 G01N30/02 

FIELD: chemistry.

SUBSTANCE: invention pertains to the quantitative method of determining thymol and carvacrol in medicinal plant raw material, in extracts and infusions of plant raw material using high-performance liquid chromatography. The result is attained by that, in the quantitative measurement of thymol and carvacrol present in the extract, a column with dimensions 250×4.6 mm is used. The column is filled with silica gel with grafted straight alkyl groups, in which the number of atoms equals sixteen (C 16), with particle size of 7 mcm. The mobile phase used is a methanol-water solution with ratio of 62:38. The speed of the mobile phase is 1 ml/min. Detection is done using a UV-detector with wavelength of 277 nm, with subsequent calculation of thymol and carvacrol relative to the area of peaks of the analysed and standard substance. The standard substance used is a standard solution of thymol-carvarol.

EFFECT: method allows for separation and quantitative measurement of content of separate thymol and carvacrol in medicinal raw material, in extracts and infusions of plant raw material, simplifies the sample preparation process, reduces the time of analysing and simplifies identification of the substance.

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