METHOD OF POSTVACCINAL IMMUNITY TO VACCINE "PNEUMO-23" ANTIGENES TEST Russian patent published in 2008 - IPC G01N33/569 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to immunology, and can be used for testing of postvaccinal immunity within vaccination by vaccine "Pneumo-23". Substance of invention consists that solid carrier with sorbate vaccine " Pneumo-23", containing 23 pneumococcus polysaccharides is covered with working cultivations of analysed and reference serum, incubated at temperature 18-22°C within 30 minutes, isolated from surplus antibodies, added with conjugate of working cultivation, repeatedly incubated at the same parameters, washed from surplus conjugate, added with substrate mixture containing tetramethylbenzidine and hydrogen peroxide of specified values, incubated at temperature 18-22°C within 15-20 minutes in lightproof place protected. Reagent is added to stop reaction. Optical density of analysed and reference serum are evaluated for antibody content prior to and after vaccination. Optical density of analysed serum increased in 4 times and more indicates postvaccinal immunity. Advantage of invention consists in increase of method sensitivity.

EFFECT: increased sensitivity of method of postvaccinal immunity test within vaccination by vaccine "Pneumo-23".

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