FIELD: medicine.
SUBSTANCE: invention relates to genetic engineering area and can be applied to medicine in diagnostics of several diseases, associated with metabolic syndrome. For determination of polymorphism Thr312Ala α-fibrinogen (FGA) gene the DNA from investigated sample is isolated and subject to amplification by polymerase chain reaction using allele-specific primers. For prevailing allele FGA T identification reverse primer 5'-TCC-CAG-AGT-TCC-AGC-TTC-CAG-T-3' is used; for rare allele FGA A identification reverse primer 5'-CCC-AGA-GTT-CCA-GCT-TCC-AGC-3' is used; as a direct primer in both cases serves the sequence 5'-TGT-CGA-GGG-TCA-TGC-AGT-AGG-G-3'. The invention makes it possible to carry out high-accuracy, simple, and low-cost analysis of fibrinogen polymorphism, suitable for clinico-diagnostic laboratories.
EFFECT: possibility to carry out high-accuracy, simple, and low-cost analysis of fibrinogen polymorphism, suitable for clinico-diagnostic laboratories.
2 dwg, 1 tbl
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