METHOD FOR OBTAINING A LINE OF HUMANISED MICE, TRANSGENIC BY hACE2 Russian patent published in 2021 - IPC C12N15/63 C12N15/85 A01K67/27 

FIELD: biotechnology.

SUBSTANCE: present invention relates to the field of biotechnology and medicine and discloses an expressed linearised vector consisting of a constitutive CAG-promoter, a STOP cassette terminating transcription from said promoter and flanked by LoxP sites, and an open frame for reading the Homo sapiens hACE2 gene, containing the full-size cDNA of hACE2, the expression whereof is induced tissue-specifically by introduction of a Cre-recombinase characterised by the fact that the cDNA of the hACE2 gene is embedded in the vector for the recognition sites of the restriction endonucleases BshTI – MluI. The invention also discloses a method for creating the described expression vector, a method for obtaining a line of mice transgenic by hACE2 using the described vector, and a transgenic mouse, tissue-specifically expressing the human angiotensin-converting enzyme-2, transformed by said vector. Due to the STOP-cassette flanked by LoxP sites, contained in the vector, the expression of the hACE2 gene can be tissue-specifically activated in the transgenic mice at any time by induction of doxycycline-dependent expression of a Cre-recombinase.

EFFECT: invention allows for creation of lines of genetically modified humanised mice for use as animal models in the research of the mechanisms of pathogenesis of COVID-19 and development of therapeutic approaches.

4 cl, 1 dwg, 7 ex