FIELD: biology.
SUBSTANCE: invention concerns molecular biology. It claims an oligonucleotide primer set for defining sturgeon species with the following nucleotide sequences: reverse primer AHR (TATACACCATTATCTCTATGT) for sturgeon species identification, and direct primers: AGF (GCACAGACTATGTGGTATCCAGAA) for identification of Russian sturgeon species, ABF (CAGATGCCAGTAACAGGCTGA) - for Russian and Siberian sturgeon, ABRM (TGTCTGTCTAGAACATAtG) - for Siberian sturgeon, HusF (TATCTATTACCTGCGAGCAGGCTG) - for hausen (white sturgeon), DauF (CCTCTTATGTACGCGGTGT) - for kaluga (great Siberian sturgeon), SchF (TGTGGGGTCACGGACTTTACAG) - for Amur sturgeon, NudF (TGTCTTTTCTGAAGGAGCTTTGC) - for barbell sturgeon, RutF (GGGAATAACCGTTAATTTGG) - for sterlet, and SteF (GGGGTTCTTGGCATGTTGTGAGCG) - for stellate sturgeon.
EFFECT: detection of presence of mitochondrial DNA belonging to one of eight sturgeon species.
1 dwg, 1 tbl, 10 ex
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