FIELD: agriculture.
SUBSTANCE: invention relates to the fishing industry and can be used in the artificial reproduction of valuable species of fish, particularly sturgeon. The method of cryopreservation of eggs of sturgeon lays in treatment with protective solutions, equilibration, freezing, thawing. Equilibration is carried out for 1.5 hours a temperature of 14-18° C, and as a protector, a mixture of sunflower oil and fish oil is used in the ratio 1:1 at a freeze rate of 1500°C/min. Thawing of the samples is carried out in distilled water at a temperature of 42-45°C for 45-75 seconds until the liquid fraction.
EFFECT: use of the claimed invention enables to improve the quality of defrosted eggs of sturgeon, as well as to create cryobanks with samples of genetic material.
1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF IMPROVEMENT OF SURVIVAL RATE OF GERMINAL CELLS OF STURGEONS AT CRYOPRESERVATION | 2009 |
|
RU2399201C1 |
| METHOD FOR CRYOGENIC PRESERVATION OF STURGEON FISH SEMEN | 2006 |
|
RU2317703C1 |
| PROTECTIVE MEDIUM FOR CRYOCONSERVATION OF STURGEON FISH SPERM | 2018 |
|
RU2683682C1 |
| METHOD FOR OBTAINING UNISEXUAL FEMALE OFFSPRING IN STURGEON FISH | 2005 |
|
RU2312495C2 |
| COMPOSITION OF BIOLOGICALLY ACTIVE PREPARATIONS FOR IMPROVING SURVIVAL RATE OF STURGEONS IN THEIR ARTIFICIAL PROPAGATION | 2010 |
|
RU2453112C1 |
| METHOD FOR CRYOGENIC PRESERVATION OF CARP FISH SPERM | 2003 |
|
RU2241324C2 |
| METHOD OF CREATION OF REPRODUCTIVE BROODSTOCKS OF STURGEONS | 2012 |
|
RU2518442C1 |
| PROTECTIVE MEDIUM FOR CRYOPRESERVATION OF STURGEON SPERM | 2023 |
|
RU2811679C1 |
| METHOD FOR INCREASING EFFICIENCY OF SPAWN REPRODUCTION AND POPULATION OF STURGEONS FISH | 2004 |
|
RU2262844C1 |
| DEVICE FOR DETERMINING THE STATE OF OOCYTES AND MATURITY STAGE OF GONADS IN STURGEON AND CARP FISHES | 2018 |
|
RU2708156C1 |
