WAY OF DIFFERENTIAL DNA-DIAGNOSTICS AT DIFFERENT STAGES OF LIFE CYCLE OF HELMINTHS-ACTIVATORS OF HUMAN AND ANIMAL FASCIOLIASIS Russian patent published in 2009 - IPC C12Q1/68 

Abstract RU 2360003 C1

FIELD: medicine.

SUBSTANCE: present invention concerns area of molecular genetics and parasitology. The way includes allocation of DNA of helminths or DNA of the infected molluscum from Lymnaeidae family, amplification of sites of mitochondrial DNA with use of three host-dependent primers. Further separation and visualisation of the amplificated fragments and identification of a specific accessory of helminths on number and the size (length) of taped polymorphic markers is performed. The way allows carrying out specific identification at different stages of life cycle of originators of a fascioliasis of animals and the person - hepatic fluke F. hepatica and F. gigantica.

EFFECT: invention can be used in the field of veterinary science and medicine.

2 dwg, 2 ex

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RU 2 360 003 C1

Authors

Semenova Serafima Konstantinovna

Vasil'Ev Vasilij Aleksandrovich

Ryskov Aleksej Petrovich

Dates

2009-06-27Published

2007-11-02Filed