FIELD: chemistry.
SUBSTANCE: claimed invention relates to new oligosaccharides modified with fluorophorous marker, namely, 4-O-methylumbelliferyl β-D-xylopiranosil-(1,4)-bis-[O-β-D-xylopiranosil-(1,4)]-β-D-xylopiranoside, 4-O methylumbelliferyl β-D-xylopiranosil-(1,4)-tris-[O-β-D-xylopiranosil-(1,4)]-β-D-xylopiranoside, 4-O-methylumbelliferyl β-D-xylopiranosil-(1,4)-tetrakis-[O-β-D-xylopiranosil-(1,4)]-β-D-xylopiranoside. It is intended for measuring activities of both pure and coarse preparations of β-xylanases. The invention also relates to fermentative synthesis of oligosaccharides with formula: 
, which lies in incubation of β-xylosidase from Aspergillus sp. at 37°C in neutral reaction medium in presence of aryl-β-D-xylopiranoside and 4-O-methylumbelliferyl-β-D-xylopiranoside.
EFFECT: possibility to measure activities of both pure and coarse preparations of β-xylanases.
4 cl, 1 dwg, 2 tbl
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