FIELD: medicine, microbiology.
SUBSTANCE: invention concerns veterinary microbiology, in particular, cultivation of tuberculosis micobacteria on liquid nutrient mediums. Crops of culture of micobacteria carry out on a surface of the stab prepared from beef-extract agar, in addition containing on 100 of gel - 1.0-1.2 g of glucose and 2.0-2.2 ml of glycerine, height 8.0-8.2 cm and diameter 1.0-1.2 cm which acts over a surface of a liquid nutrient medium on 2-3 mm. For definition of diagnostic informativity of the given way of micobacteria cultivation, carry out seeding of reference strains of cultures of tuberculosis micobacteria: M.bovis (un. 8, IEV); M.tuberculosis (uit. H37Rv), M.avium (piece 780, IEV) M.smegmatis (cultures IEVSaFE). The invention allows to accelerate for 2-6 days primary and intensive growth of micobacteria of a tuberculosis in comparison with other ways of crops and promotes thus to the maximum accumulation of bacteriemic mass of cultures of micobacteria of a tuberculosis that testifies about its diagnostic informativity.
EFFECT: acceleration for 2-6 days of primary and intensive growth of tuberculosis micobacteria in comparison with other ways of crops and maintenance of the maximum accumulation of bacteriemic mass of cultures of tuberculosis micobacteria.
2 cl, 2 tbl, 6 ex
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