FIELD: biotechnology.
SUBSTANCE: the present innovation deals with inoculating lactic-acid bacteria or enterobacteria onto the inferior layer of nutritive medium being a beef-extract agar, applying the superior layer of nutritive medium that contains disubstituted potassium phosphate, magnesium sulfate, L-asparagin, glycerol, citric acid, citric-acid ammonium iron, humivit, agar, aqueous solution of hen egg's yolk and distilled water onto the inferior layer, the inoculation of the superior layer of nutritive medium with a culture of indicator strain of mycobacteria followed by detecting antagonistic activity which should be evaluated according to the quantity of colony-forming units (CFU) inhibiting the growth of 1-5·103 CFU of indicator strain of mycobacteria/ml suspension. The innovation enables to detect antagonistic activity of lactic-acid bacteria and enterobacteria to pathogenic mycobacteria.
EFFECT: higher accuracy of detection.
2 ex, 2 tbl
| Title | Year | Author | Number |
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| METHOD OF PROBIOTIC ANALYSIS FOR ANTAGONISTIC ACTIVITY CONCERNING LYOPHILISED AEROBIOTIC BACTERIA BIOMASS IN RELATION TO PATHOGENIC MYCOBACTERIA | 2007 |
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| METHOD OF DETERMINING ANTAGONISTIC ACTIVITY OF BIFIDUS BACTERIA RELATIVE TO MICOBACTERIUM AND RHODOCOCCUS | 2007 |
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| METHOD FOR ASSESSING ANTAGONISTIC PROPERTIES OF INTESTINAL BACTERIA TOWARDS PATHOGENIC MICOBACTERIA | 2006 |
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| METHOD OF ESTIMATION OF PROBIOTICS OPPOSING ACTIVITY ON BASIS OF LYOPHILISED BIOMASS OF ANAEROBIC BACTERIA IN RELATION TO PATHOGENIC MICOBACTERIA | 2007 |
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| METHOD FOR DETECTING ANTAGONISTIC PROPERTIES WITH RESPECT TO PATHOGENIC MYCOBACTERIUM IN LACTIC ACID MICROORGANISMS AND MICROORGANISMS OF ESCHERICHIA COLI GROUP | 2006 |
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| DENSE GROWTH MEDIUM FOR CULTIVATION OF PATHOGENIC MYCOBACTERIA AND NOCARDIOFORM RAY FUNGI | 2008 |
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| STRAIN OF Lactobacillus fermentum Z, USED FOR PRODUCTION OF PROBIOTIC CULTURED MILK FOODS | 2008 |
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| NUTRIENT MEDIUM FOR ISOLATION AND CULTURING MYCOBACTERIA FROM BIOLOGICAL MATERIAL | 2006 |
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| METHOD FOR DETERMINING SENSITIVITY OF CAMPYLOBACTERIAE TO PROBIOTICS AND AUTOPROBIOTICS | 2022 |
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RU2816763C1 |
