METHOD OF DETERMINING QUANTITATIVE COMPOSITION OF MULTI-COMPONENT MEDICINAL PREPARATION WITH ANTITHERMIC AND ANTIALLERGIC ACTION Russian patent published in 2008 - IPC G01N33/15 G01N30/00 G01N30/34 G01N30/36 

FIELD: chemistry.

SUBSTANCE: present invention pertains to analytical chemistry, and particularly to methods of determining the quantitative composition of multi-component medicinal preparations with antithermic and antiallergic action. The method is achieved through inversed-phase high-efficiency liquid chromatography with an ultraviolet spectrophotometric detector and a chromatograph column, filled with a Zorbax SB C8 sorbent, in a linear concentration gradient mode of acetonitrile in a mobile phase during analysis. The mobile phase is a mixture of acetonitrile and a phosphate buffer solution. The content of defined components is calculated from the surface area of peaks on the chromatograms of the test solution and solution of working standard samples. With the objective of efficient separation of peaks of all active and auxiliary substances, the content of the mobile phase during analysis is changed from a phosphate buffer solution with pH 3.0 to a mixture of acetonitrile with a phosphate buffer solution with pH 6.8 in volume ratio of 1:4.

EFFECT: design of an efficient method of determining quantitative composition of multi-component medicinal preparations with antithermic and antillergic action.

1 ex, 1 tbl, 7 dwg