FIELD: medicine; pharmacology.
SUBSTANCE: labeling method of mononuclear cells of red bone marrow by means of 1 99mTs-exametazime, characterised by reception of cellular suspension, incubation of cellular suspension about 4 ml 99mTs-eksametazima during 10 minutes is declared at room temperature, with the subsequent washing, centrifugation of 5 minutes at 150 g, the collecting of supernatant fraction, resuspending of deposit containing labeled 99mTs-exametazim mononuclear cells of bone marrow, thus incubation is performed with 1500-2000 MLL 99mTs-exametazim, washing is performed in 10 ml of an isotonic solution of sodium chlorine, and resuspending in 10 ml of isotonic solution of sodium chlorine.
EFFECT: possibility to mark mononuclear cells of red bone marrow and to avoid fence of additional quantity of blood for the purpose of reception of cell-free plasma for washing of cells.
1 ex, 1 dwg
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Authors
Dates
2009-01-20—Published
2007-05-28—Filed