METHOD FOR INFLAMMATION FOCI IDENTIFICATION BY THE METHOD OF POLY-ORGANIC SCINTIGRAPHY Russian patent published in 2018 - IPC A61B6/03 A61K49/04 A61P43/00 

FIELD: medicine.

SUBSTANCE: invention relates to the field of medicine, namely, to radionuclide diagnostics and can be used to identify inflammation foci using the multi-organ scintigraphy technique. Performing the blood sampling of at least 250–400 ml into the first sterile bag, which is centrifuged at a speed of 800 rpm for the time required to precipitate the red blood cells. Enriched with leukocytes supernatant plasma is transferred into the second bag. Contents of the second bag is centrifuged at a speed of 1,300 rpm for the time required to obtain auto-leukocytes. Then, the supernatant plasma from the second bag is transferred to the third bag, and the red cells from the first bag are intravenously returned to the patient. Adding the radiopharmaceutical preparation (RFP) in an amount of 3–5 ml into the remaining in the second bag auto-leukocytes. Resulting cells suspension is incubated for 8–10 minutes. To this, plasma from the third bag is added in an amount of 10–20 ml, followed by centrifugation of the second bag with the resulting cells suspension at a speed of 2,000 rpm for the time necessary to separate the labeled auto-leukocytes from free RFP that has not been bound to leukocytes. Removing the free RFP from the second bag. Adding the remaining plasma into labeled auto-leukocytes and from the third bag and resuspending to obtain a preparation that is administered to the patient for multi-organ scintigraphy.

EFFECT: method provides an increase in the reliability of the inflammation foci determination, that is, obtaining more objective results of the study, due to using a blood cells preparation characterized by the absence of free radioactivity and impurities of labeled auto-erythrocytes, while maintaining functional properties of the cellular elements, as well as a qualitative evaluation of the labeled leukocytes accumulation in the proposed zone of inflammation by visual and quantitative determination of the RFP increased accumulation area.

1 cl, 10 dwg, 8 ex