FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions relates to biotechnology. Claimed is cell of mycelial fungus Penicillium canescens with removed catabolite repression and arabinose induction, producing xylanase and laccase. Cell is transformed with plasmid, containing promoter of gene bgaS, bgaS leader peptide, xylanase-coding DNA fragment, bgaS terminator, β-lactamase gene and pMB1 replicon, and plasmid, which contains promoter of gene bgaS, bgaS leader peptide, laccase-coding DNA fragment, bgaS terminator, β-lactamase gene and pMB1 replicon. Also claimed is method of enzyme preparation of xylanase and laccase with application of said cell of Penicillium canescens.
EFFECT: group of inventions provides increased output of target enzymes.
10 cl, 9 dwg, 3 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT LACCASE OF LIGNINOLYTIC FUNGUS Trametes sp AND METHOD OF ITS OBTAINING | 2008 |
|
RU2394912C2 |
| DNA FRAGMENT OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM ENCODING SYNTHESIS OF SECRETABLE ENDOGLUCANASE III AND GROUP OF PENICILLIUM CANESCENS STRAINS PRODUCING PENICILLIUM VERRUCULOSUM ENDOGLUCANASE III CONSTRUCTED BY METHODS OF TRANSFORMATION AND GENETIC ENGINEERING BASED ON THIS DNA FRAGMENT | 2001 |
|
RU2238974C2 |
| METHOD OF PRODUCING ENZYMATIC AGENT FOR DEGRADATION OF HEMICELLULOSE HETEROPOLYSACCHARIDES OF HERBAL CYTODERM AND ENZYMATIC AGENT (VERSIONS) | 2007 |
|
RU2358756C1 |
| NOVEL ENDO-(1-4)β-D-XYLANASE FROM PENICILLIUM CANESCENS (VERSIONS), DNA FRAGMENT, CODING SECRETED ENDO-(1-4)β-D-XYLANASE FROM PENICILLIUM CANESCENS (VERSIONS) AND METHOD OF OBTAINING IT | 2009 |
|
RU2412246C2 |
| DNA PCX-302 FRAGMENT ENCODING SYNTHESIS OF PENICILLIUM CANESCENS SECRETED ENDO-(1-4)-BETA-XYLANASE AND FUNGUS STRAIN PENICILLIUM CANESCENS AS PRODUCER OF SECRETED ENDO-(1-4)-BETA-XYLANASE | 2001 |
|
RU2197526C1 |
| METHOD FOR PREPARING FODDER COMPLEX ENZYME PREPARATION (VARIANTS) AND STRAIN PENICILLIUM CANESCENS (VARIANTS) | 2004 |
|
RU2288267C2 |
| RECOMBINANT STRAIN OF MITSENAL MUSHROOM PENICILLIUM CANESCENS CL14, PRODUCTION COMPONENT OF CELLULOSUM CLOSTRIDIUM THERMOCELLUM AND METHOD OF ITS CULTIVATION | 2016 |
|
RU2646132C1 |
| NEW RECOMBINANT STRAIN OF FILAMENTOUS FUNGI PENICILLIUM CANESCENS CS15, PRODUCING CELLULASE CLOSTRIDIUM THERMOCELLUM AND METHOD OF ITS CULTIVATION | 2015 |
|
RU2612158C1 |
| GENETIC MAKER TO ENSURE EXPRESSION OF TARGET HOMOLOGOUS AND HETEROLOGOUS GENES IN CELLS OF FILAMENTOUS FUNGUS PENICILLIUM VERRUCULOSUM; USED AS HOST, METHOD FOR MAKING STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR MAKING ENZYMATIC AGENT | 2008 |
|
RU2378372C2 |
| DNA PCG-2,6 FRAGMENT ENCODING SYNTHESIS OF SECRETED BETA-GALACTOSIDASE OF PENICILLIUM CANESCENS AND THE STRAIN OF FUNGUS PENICILLIUM CANESCENS - PRODUCER OF SECRETED BETA-GALACTOSIDASE | 1997 |
|
RU2126049C1 |
