METHOD OF DETERMINING ANTAGONISTIC ACTIVITY OF BIFIDUS BACTERIA RELATIVE TO MICOBACTERIUM AND RHODOCOCCUS Russian patent published in 2009 - IPC C12Q1/00 

FIELD: chemistry, biochemistry.

SUBSTANCE: proposed method comprises adding bifidus bacteria into Blaurock medium, cultivation at a temperature and time optimum, agar-processing by agar melted in a physiological solution and cooled down to 70°C. A nutrient medium is applied onto agar-processed medium with grown bifidus bacteria, the aforesaid nutrient medium containing 0.4 g of L-asparagine, 0.05 g of disubstituted potassium phosphate, 0.05 g of magnesium sulphate, 0.2 g of citric acid, 0.005 g of ammoniac iron-citrate, 0.05 g of sodium pyruvate, 4.0 g of glycerin, 3.0 g of agar, 10.0 ml of humivite, 10.0 ml of water solution of an egg yolk (1:1), and about 100.0 ml of distilled water. The indicator strain culture is sown on the aforesaid preparation and is incubated for 14 to 30 days. The results are estimated from the growth blocking index.

EFFECT: provision of optimum conditions for growth of strain under analysis and indicator culture growth.

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