FIELD: chemistry.
SUBSTANCE: invention concerns microbiology and can be applied in detection of antagonistic properties towards pathogenic micobacteria. The method involves seeding intestinal bacteria to lower layer of nutrient medium containing double-substituted potassium phosphate, magnesium sulfate, L-asparagine, glycerin, citric acid, ammoniac iron citrate, humivit, agar and distilled water; heating on bain-marie at 100°C for 5-10 minutes; skewing at room temperature; application of the aforesaid composition to the lower layer; seeding indication micobacteria strain to the upper layer with further cultivation; detection of antagonistic activity by the ratio of indication strain colonies grown on thick nutrient agar to the number of indication strain colonies grown in double-layer medium.
EFFECT: increased efficiency of method for assessing antagonistic properties towards pathogenic micobacteria.
3 tbl, 2 ex
| Title | Year | Author | Number |
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| NUTRIENT MEDIUM FOR ISOLATION AND CULTURING MYCOBACTERIA FROM BIOLOGICAL MATERIAL | 2006 |
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| SOLID NUTRIENT MEDIUM FOR MYCOBACTERIUM CULTURING | 2006 |
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RU2320716C2 |
| NUTRIENT MEDIUM FOR CULTURING MYCOBACTERIUM AND NOCARDIOFORMOUS ACTINOMYCES | 2006 |
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RU2322495C2 |
| METHOD FOR DETECTING ANTAGONISTIC PROPERTIES WITH RESPECT TO PATHOGENIC MYCOBACTERIUM IN LACTIC ACID MICROORGANISMS AND MICROORGANISMS OF ESCHERICHIA COLI GROUP | 2006 |
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RU2320724C1 |
| METHOD FOR DETERMINING ANTAGONIST ACTIVITY OF PROBIOTICS FROM SPORE BACTERIA, AGAINST MICOBACTERIA | 2007 |
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