METHOD FOR ASSESSING THE GENETIC POTENTIAL OF MANYCH MERINO SHEEP BASED ON MOLECULAR GENETIC MARKERS Russian patent published in 2022 - IPC A01K67/02 

FIELD: biotechnology.

SUBSTANCE: The invention relates to the field of biotechnology. The substance of the proposed method for assessing the genetic potential of Manych Merino sheep based on molecular genetic markers is as follows: DNA is isolated, a fragment of the GH, CAST and GDF9 genes is amplified by PCR-RFLP (restriction fragment length polymorphism) using primers: GH - (F : 5'-GGA-GGC-AGG-AAG-GGA-TGA-A-3' and R: 5'-CCA-AGG-GAG-GGA-GAG-ACA-GA-3'); CAST - (F: 5'-TGG-GGC-CCA-ATG-ACG-CCA-TCG-ATG-3' and R: 5'-GGT-GGA-GCA-CTT-CTG-ATC-ACC-3'); GDF9 (F: 5'-GAA-GAC-TGG-TAT-GGG-GAA-ATG-3' and R: 5'-CCA-ATC-TGC-TCC-TAC-ACA-CCT-3'); restriction of the amplified GH gene fragment with HaeIII endonuclease, CAST MspI endonuclease, GDF9 gene with BstHHl restriction endonuclease; identification of genotypes and selection of animals with desired complex genotypes.

EFFECT: invention makes it possible to evaluate the genetic potential of Manych Merino sheep based on molecular genetic markers and can be used for in vivo evaluation of the high meat productivity of Manych Merino sheep based on the identification of genetic markers associated with growth indicators.

1 cl, 9 dwg