FIELD: medicine.
SUBSTANCE: what is presented is a method for genotyping TXNRD1 rs 1128446 gene polymorphism providing conducting PCR with using specially specified pair of primers (F: 5'-GGCTTCTCGTAGCCATTAGG-3' and R: 5'-TATCTTCCCTTCCCCGAGAC-3') that is followed by the analysis of the PCR product by RFLP which involves Taq1 endonuclease processing and fractionation of the produced fragments in 2% agarose gel. The DNA fragments sized 179 and 83 base pairs are verified as a wild type CC homozygous genotype, the fragment sized 262 base pairs - as a homozygous mutant genotype GG, while the fragments 262 sized 179 and 83 base pairs - as a heterozygous genotype CG.
EFFECT: method is characterised by diagnostic simplicity and accuracy.
1 dwg, 1 ex
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