MOLECULAR GENETIC MARKER OF CAPS TYPE IN PEA PLANTS PISUM SATIVUM LINNAEUS AND METHOD FOR IDENTIFYING PLANTS HOMOZYGOUS FOR ALLELE, WHICH CAUSES FORMATION OF HIGH SPECIFICITY WITH RESPECT TO SYMBIOTIC NODULE BACTERIA Russian patent published in 2024 - IPC C12N15/82 A01H1/04 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology, specifically to a CAPS type molecular marker for identifying Pisum sativum L. plants homozygous for the PsLykX^A allele. Invention also relates to use of said marker for use in selection of pea plants. To implement the pea plants identification method, pea plants DNA is amplified using a pair of primers A1B1 or A2B2, which are part of a molecular marker, hydrolysis of the amplification product is carried out using the restriction endonuclease Hpy188III, which is part of the molecular marker, and the number of hydrolysed amplification products and their size are determined. During hydrolysis of the product amplified using a pair of primers A1B1, in case of detecting two fragments of size 217 bp and 164 bp, a plant of seed peas Pisum sativum L. is identified as homozygous for allele PsLykX^A. During hydrolysis of the product amplified using a pair of primers A2B2, if two fragments of 127 bp and 81 bp are detected, the Pisum sativum L. pea plant is identified as homozygous for the PsLykX^Aallele.

EFFECT: invention increases efficiency, speed and accuracy of identification, as well as simplifies identification of plants of peas Pisum sativum Linnaeus, homozygous for allele PsLykX^A, causing formation of high specificity in relation to symbiotic nodule bacteria.

5 cl, 5 dwg, 1 tbl, 5 ex