METHOD OF FLAX SEED ASSESSMENT FOR FREE RADICALS BY CHEMILUMINESCENCE METHOD Russian patent published in 2009 - IPC G01N33/02 

Abstract RU 2350949 C1

FIELD: chemistry.

SUBSTANCE: invention refers to fat-and-oil industry. Test subject is flax seed extract produced from flax grinded to particle size 0.01-0.5 mm, mixed with phosphate buffer of pH=7.5 in ratio 1:10 within 1 hour 30 minutes. Then filtrate that is flax extract, is centrifuged at rotor speed 6000 min-1 within 30 minutes to be separated. Extract is analysed, therefore a measuring cell is filled with 0.1 ml extract, 0.4 ml phosphate buffer (pH=7.5), 0.4 ml ferric sulphate (II) solution, 0.2 ml hydrogen peroxide. Herewith kinetic curve slope is considered to describe stopping power of the same product. The greater maximum chemoluminescence intensity is, the more free radicals are in flax seed.

EFFECT: possibility to develop more exact and express method ensuring timely detection of processes causing flax seed damage in storage.

1 dwg, 1 tbl, 1 ex

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RU 2 350 949 C1

Authors

Shevtsov Aleksandr Anatol'Evich

Alekseeva Tat'Jana Vasil'Evna

Frolova Larisa Nikolaevna

Chernikova Ekaterina Aleksandrovna

Eremenko Nadezhda Viktorovna

Dates

2009-03-27Published

2007-12-03Filed