FIELD: medicine. SUBSTANCE: method involves preparing patient blood samples to be studied, and reagents like iron sulfate, luminol, phosphate buffer with pH equal to 7.5, mixing the samples and the reagents, recording noise signals and chemiluminescence of the produced mixture with optical sum of chemiluminescence determined. Citrated blood plasma containing no blood platelets is used as blood fraction under study. Iron sulfate solution is prepared by dissolving the weighed portion in boiling water. The solution is centrifuged in hermetically sealed reservoir with following transfer of the supernatant into the other test tube and placing the supernatant under the chemically inert oil layer. Chemiluminescence peak amplitude is determined for evaluating intensity of blood lipid peroxidation processes. Mixture components are introduced into the mixture in the following order. First of all, plasma under study is introduced into the buffer solution, then iron sulfate solution is added and hydrogen peroxide solution is introduced after switching in chemiluminescence signal recording mode. Conditions for carrying out the method is as follows. Hydrogen peroxide solution is introduced into the cell under darkness conditions using metering device after placing the cell into light impermeable measurement chamber of chemiluminometer. The metering device has cap from light insulation material on the end piece. The metering device is not removed from measuring chamber socket until the reaction is not over. Phosphate buffer is prepared to chemiluminescence reaction by means of centrifuging the prepared buffer and transferring supernatant into another reservoir. EFFECT: high accuracy of measurements. 5 cl
