FIELD: microbiology, pharmacology.
SUBSTANCE: invention relates to field of microbiology ad pharmacology. Claimed is novel recombinant version of polypeptide PapM. Polypeptide possesses activity of SAM-dependent N-metyhyltransferase and is able to catalyse reaction of methylating 4-amino-L-phenylalanine to 4-methylamino-L-phenylalanine with formation of majoritarian isoform PIB streptogramin B. Polypeptide is obtained by at least one substitution of glycine in position 249 with serine and/or substitution of threonine in position 192 with isoleucine in sequence of polypeptide ParM of wild type S. Pristinaespiralis. Also claimed are nucleic acid encoding it, vector, containing said nucleic acid, host cell, method of obtaining polypeptide, strains, producing it.
EFFECT: invention can be used in medicine.
37cl, 13 dwg, 9 tbl, 9 ex
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