DNA MUTAGENESIS BY DISORDERED FRAGMENTATION AND SECONDARY ASSEMBLY Russian patent published in 2000 - IPC

FIELD: genetic engineering, molecular biology. SUBSTANCE: matrix double-stranded polynucleotide is cleft for double-stranded disordered fragments. To obtained population oligonucleotides containing identity and heterology site with matrix polynucleotide are added followed by denaturation to single-stranded fragments and incubation in the presence of polymerase under conditions resulting to formation of mutant double-stranded polynucleotides. Chimeric polynucleotide is prepared by disordered fragmentation of different matrix polynucleotides containing sites of identity and heterology followed by denaturation and incubation with polymerase. Invention ensures to develop methods of operation of molecular evolution of complex linear sequences by disordered recombination procedure using. Invention can be used for preparing polynucleotides with required properties. EFFECT: improved method of DNA mutagenesis. 19 cl, 13 dwg, 8 tbl, 13 ex