METHOD OF PRODUCING ANTIGEN ERYTHROCYTIC PASTEURELLOSIS DIAGNOSTICUM Russian patent published in 2009 - IPC A61K39/00 G01N33/531 G01N33/543 

FIELD: veterinary science.

SUBSTANCE: invention refers to veterinary microbiology and biotechnology can be used for development of specific diagnostic aids. According to the invention the method covers producing antigen erythrocytic pasteurellosis diagnosticum by extracting pasteurellosis capsular antigen from Pasteurella cultures with sodium chloride brine, with centrifuging the extract and separating the supernatant exposed to sterilisation filtration and used for sensitisation of formalinised anionised animal erythrocytes. Pasteurellosis capsular antigen is extracted with 2.0-2.5% sodium chloride brine at 40-42°C during 30-40 min. Prior to sterilisation filtration, the supernatant is heated up at 65-70°C within 15-30 minutes.

EFFECT: application of the method allows for high-quality end product due to improved sensitivity and specificity.

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