FIELD: medicine.
SUBSTANCE: invention refers to medicine and biotechnology and concerns antigen-containing liposome preparation process. Substance of the invention includes mixing the dissolved H and D fractions of pseudocholera agent antigens, dissolving the neutral lipids in chloroform, blending the mixed dissolved antigen and lipids, preparing the liposomes by phase-inversion evaporation, removing unenclosed material vesicles by dialysis thereby using phosphate buffer 0.01 M, pH 7.2. The prepared liposomes contain 89-93% H and D fractions of pseudocholera agent antigens of size 600-800 nm.
EFFECT: advantage of the invention consists in liposome preparation of high load of pseudocholera antigen.
2 ex, 2 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING LIPOSOME WITH ENCAPSULATED DOXYCYCLINE HYDROCHLORIDE AND SURFACE LOCALISED MONOCLONAL ANTIBODIES | 2015 |
|
RU2582969C1 |
| METHOD FOR PRODUCTION OF COMBINED LIPOSOME ANTIBACTERIAL PREPARATION | 2008 |
|
RU2376012C1 |
| METHOD FOR IMMUNOPROPHYLAXIS OF EXPERIMENTAL PSEUDOCHOLERA WITH ENTRAPPED ANTIGENS Burkholderia pseudomallei | 2008 |
|
RU2373955C1 |
| METHOD OF PREPARING PROTECTIVE GLYCOPROTEIN OF MELIOIDOSIS PATHOGEN | 1996 |
|
RU2109520C1 |
| GRANZYME B DELIVERY METHOD TO MAMMALIAN CELLS | 2021 |
|
RU2796685C2 |
| METHOD FOR THE DETERMINATION OF LIPOPOLYSACCHARIDE ANTIGENS OF MICROORGANISMS IN BLOOD SERUM | 0 |
|
SU1720003A1 |
| DOSAGE FORM OF SPECIFIC IMMUNOBIOLOGICAL MEDICINE FOR TREATING AND PREVENTING HIV INFECTION AND PREPARATION METHOD THEREOF | 2014 |
|
RU2600031C2 |
| METHOD OF DETERMINATION OF ANTI-BODIES TO RE GLYCOLYPIDES | 0 |
|
SU1141336A1 |
| METHOD FOR DETERMINING LEVEL OF TOXICANTS IN WATER, FOOD PRODUCTS OR PHYSIOLOGICAL LIQUIDS, AND TEST SYSTEM | 2012 |
|
RU2508553C1 |
| METHOD FOR INCREASING ANTIGEN IMMUNOGENECITY OF B. pseudomallei ANTIGENS IN EXPERIMENTAL MELIOIDOSIS | 2012 |
|
RU2483752C1 |
