FIELD: medicine; biotechnology.
SUBSTANCE: sea water salinity is defined in the area of bioluminescent bacteria separation. Nutrient medium based on distilled water is prepared with addition of sodium chloride in amount corresponding to defined salinity value, and dry nutrient agar in amount of 34.0-36.0 g per litre of distilled water. Bioluminescent bacteria are separated from sea water by membrane filter method, inoculated to nutrient medium and cultivated at room temperature for 22-24 hours. Presence of bioluminescent bacteria is defined by bioluminescence displayed by bacteria.
EFFECT: enhanced output of bioluminescent bacteria, reduced cost of nutrient medium.
1 tbl, 6 ex
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Authors
Dates
2009-06-10—Published
2007-04-16—Filed