FIELD: veterinary.
SUBSTANCE: invention relates to molecular biology and veterinary medicine and can be used for control of anti-epizootic measures in case of farm animal and poultry salmonellosis. Method includes: selection of biomaterial, DNA isolation, detecting genome DNA of Salmonella genus microorganism in PCR using specially selected primers; for detecting genome DNA of Salmonella genus microorganisms in PCR primers with the following nucleotide sequences are used: 5'-GAGCATATTCGTGGAGCAATG-3' and 5'-AATAACATCCTCAACTTCAGCAG-3 and fluorescent probe Taqman FAM-TGCTCGTAATTCGCCGCCATTGG-suppressor.
EFFECT: method ensures high sensitivity and specificity to microorganisms of genus Salmonella, which allows to detect presence of latter in investigated samples reliably and quantitatively.
2 cl, 4 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING SALMONELLA (SALMONELLA SPP) DNA IN BIOLOGICAL MATERIAL OF ANIMALS, FOOD AND ANIMAL AND VEGETABLE FODDERS | 2018 |
|
RU2700476C1 |
| METHOD OF QUALITY CONTROL OF CHICKEN VACCINATION AGAINST MAREK'S DISEASE | 2006 |
|
RU2332235C2 |
| TEST SYSTEM FOR DETECTING SALMONELLA (SALMONELLA SPP.) DNA IN ANIMAL BIOLOGICAL MATERIAL, FOOD PRODUCTS AND ANIMAL AND VEGETABLE FODDERS | 2018 |
|
RU2700247C1 |
| METHOD OF DETECTING ORNITHOBACTERIOSIS IN POULTRY | 2009 |
|
RU2407801C1 |
| OLIGONUCLEOTIDES AND METHOD OF DETERMINING DNA OF BACTERIA BELONGING TO FAMILY Chlamydiaceae | 2011 |
|
RU2486255C1 |
| KIT FOR DETECTING PATHOGENIC MICROORGANISMS OF LISTERIA MONOCYTOGENES TYPE AND METHOD FOR DETECTION THEREOF IN SAMPLES OF BIOMATERIAL, IN SAMPLES OF FODDERS, IN OBJECTS OF EXTERNAL ENVIRONMENT | 2019 |
|
RU2730658C1 |
| METHOD OF DECTING PASTEURELLA AND/OR Pseudomonas Aeruginosa bacteria | 2008 |
|
RU2391409C1 |
| METHOD FOR INCREASE OF REAL TIME POLYMERASE CHAIN REACTION SENSITIVITY IN DETECTION OF PATHOGENIC BACTERIA DNA | 2016 |
|
RU2636458C1 |
| METHOD FOR IDENTIFICATION OF VIBRIO-CHLERAE O1 STRAINS, DETERMINATION OF THEIR TOXIGENICITY AND BIOVAR WITH DIFFERENTIATION OF EL TOR BIOVAR ON TYPICAL AND GENETICALLY CHANGED VERSIONS BY MULTIPLEX POLYMERASE CHAIN REACTION AND TEST SYSTEM FOR IMPLEMENTATION THEREOF TAKING INTO ACCOUNT RESULTS IN REAL TIME MODE | 2019 |
|
RU2732448C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES AND METHOD FOR DETECTING DNA OF AGENTS OF GLANDERS AND MELIOIDOSIS BY PCR METHOD WITH PRODUCT DETECTION IN REAL TIME | 2019 |
|
RU2738358C1 |
