FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to veterinary microbiology and biotechnology and specifically to methods of determining genomic DNA of bacteria. Proposed method of determining genomic DNA of Pasteurella and/or P.aeruginosa bacteria involves collection of samples and analysing the biomaterial via a polymerase chain reaction simultaneously with two pairs of primers. Further, the polymerase chain reaction products which are specific for Pasteurella and P.aeruginosa are separated through eletrophoresis.
EFFECT: invention enables detection of Pasteurella and/or Paeruginosa bacteria.
3 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETECTING ORNITHOBACTERIOSIS IN POULTRY | 2009 |
|
RU2407801C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD TO DETECT DNA OF VIRUS OF INFECTIOUS ANAEMIA OF CHICKENS WITH SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION IN REAL-TIME MODE | 2011 |
|
RU2481403C1 |
| METHOD OF QUALITY CONTROL OF CHICKEN VACCINATION AGAINST MAREK'S DISEASE | 2006 |
|
RU2332235C2 |
| METHOD OF DETECTING AND GENOTYPING BACTERIA Pasteurella multocida OF CATTLE OF SEROGROUPS A, B, D, E, F IN MULTIPLEX POLYMERASE CHAIN REACTION | 2014 |
|
RU2551962C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF STRAINS AND ISOLATES OF BACTERIA Pasteurella multocida OF SEROGROUP A IN CATTLE AND METHOD OF THEIR APPLICATION | 2013 |
|
RU2527153C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND USING THEM IN METHOD FOR IDENTIFYING SEROGROUP D Pasteurella multocida BACTERIAL STRAINS AND ISOLATES IN CATTLE BY POLYMERASE CHAIN REACTION | 2014 |
|
RU2562167C1 |
| METHOD TO DETECT PATHOGENIC STRAINS AND ISOLATES OF BACTERIA PASTEURELLA MULTOCIDA | 2012 |
|
RU2477321C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES FOR IDENTIFICATION AND SUBTYPING OF BACTERIAL DNA OF Pasteurella multocida OF SEROTYPES A, B, D, E, F BY PCR METHOD IN REAL TIME | 2014 |
|
RU2551958C1 |
| METHOD OF DETECTING MICROORGANISMS OF GENUS SALMONELLA | 2008 |
|
RU2360004C1 |
| OLIGONUCLEOTIDE PRIMERS FOR β LACTAMASE GENE DETECTON AND TYPING OF PATHOGENIC BURKHOLDERIA | 2011 |
|
RU2474614C1 |
