FIELD: medicine.
SUBSTANCE: method involves biomaterial sampling, DNA recovery and PCR-based genomic Ornithobacterium rhinotracheale DNA detection with using specially selected primers and reaction products detection. For genomic DNA detection, the PCR procedure uses two primers: upstream 5'-TGGGATTACCGCAAAATACC-3' and downstream 5'-AAACGGAGTGGTTACGCTCA-3. If observing an amplification DNA fragment sized 142 base pairs, the genomic Ornithobacterium rhinotracheale DNA is concluded to be present in the analysed sample.
EFFECT: method provides high sensitivity and specificity to Ornithobacterium rhinotracheale microorganisms that allows reliable detection of the presence of the latter in the analysed sample.
2 cl, 2 tbl, 3 ex
