FIELD: medicine; microbiology.
SUBSTANCE: liquid nutrient medium containing L-arginine, L-cysteine, L-histidine, L-isoleucine, DL-methionine, DL-leucine, L-lysine, the L-proline, DL-threonine, L-tyrosine, L-valine, L-asparaginic acid, L-serine, glucose, sodium chloride, magnesium sulphate, thiamine, calcium pantothenate, twice-substituted sodium phosphate, monosubstituted potassium phosphate, distilled water at a parity of phases 1:1, is layered on sterile perfluorodecalin, representing the lower phase. Inoculation of F. fcularensis is performed on border of the phases. Propagation of F. tularensis in a liquid diphasic nutrient medium is carried out in static conditions during 120 h at 37°C temperature.
EFFECT: increase of an output of cells of a microorganism.
1 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NUTRIENT MEDIUM FOR SUBMERGED CULTIVATION OF TULAREMIA MICROBE | 2013 |
|
RU2518282C1 |
| SYNTHETIC NUTRIENT MEDIUM FOR IMPREGNATION OF POLYACRYLAMIDE GEL CONTAINING 0 | 0 |
|
SU1818340A1 |
| LIQUID CULTURE MEDIUM FOR CULTURING MYCOBACTERIA FROM LEPROMA FROM LEPROSY PATIENTS | 2009 |
|
RU2403282C1 |
| DENSE NUTRIENT MEDIUM FOR CULTIVATION OF MYCOBACTERIA, ISOLATED FROM LEPROMAS OF LEPROSY PATIENTS | 2009 |
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RU2413764C1 |
| METHOD FOR ASSESSING CLINICAL EFFICACY OF ANTIBACTERIAL PREPARATIONS FOR SPECIAL DANGER INFECTIOUS AGENTS Francisella tularensis AND Brucella spp | 2009 |
|
RU2417376C2 |
| METHOD OF PRODUCING TULAREMIC MICROBE BIOMASS | 2010 |
|
RU2451743C2 |
| METHOD OF PRODUCTION OF CONCENTRATE OF MICROBIAL CELLS FOR OBTAINING LIVE TULAREMIA VACCINE | 2013 |
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RU2528878C1 |
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RU2822665C2 |
| SYNTHETIC NUTRIENT MEDIUM FOR BRUCELLA CULTURING | 1998 |
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RU2148638C1 |
| NUTRIENT MEDIUM FOR LEGIONELLA PNEUMOPHILA CULTIVATION | 1989 |
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RU1614494C |
