WAY TULAREMIA ORIGINATOR PROPAGATION Russian patent published in 2009 - IPC C12N1/20 C12Q1/04 

Abstract RU 2361916 C1

FIELD: medicine; microbiology.

SUBSTANCE: liquid nutrient medium containing L-arginine, L-cysteine, L-histidine, L-isoleucine, DL-methionine, DL-leucine, L-lysine, the L-proline, DL-threonine, L-tyrosine, L-valine, L-asparaginic acid, L-serine, glucose, sodium chloride, magnesium sulphate, thiamine, calcium pantothenate, twice-substituted sodium phosphate, monosubstituted potassium phosphate, distilled water at a parity of phases 1:1, is layered on sterile perfluorodecalin, representing the lower phase. Inoculation of F. fcularensis is performed on border of the phases. Propagation of F. tularensis in a liquid diphasic nutrient medium is carried out in static conditions during 120 h at 37°C temperature.

EFFECT: increase of an output of cells of a microorganism.

1 tbl, 8 ex

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Authors

Alekseev Sergej Mikhajlovich

Darmov Il'Ja Vladimirovich

Bakulin Mikhail Konstantinovich

Grudtsyna Anna Stanislavovna

Pletneva Anna Jur'Evna

Dates

2009-07-20Published

2007-11-01Filed