FIELD: medicine; microbiology.
SUBSTANCE: liquid nutrient medium containing L-arginine, L-cysteine, L-histidine, L-isoleucine, DL-methionine, DL-leucine, L-lysine, the L-proline, DL-threonine, L-tyrosine, L-valine, L-asparaginic acid, L-serine, glucose, sodium chloride, magnesium sulphate, thiamine, calcium pantothenate, twice-substituted sodium phosphate, monosubstituted potassium phosphate, distilled water at a parity of phases 1:1, is layered on sterile perfluorodecalin, representing the lower phase. Inoculation of F. fcularensis is performed on border of the phases. Propagation of F. tularensis in a liquid diphasic nutrient medium is carried out in static conditions during 120 h at 37°C temperature.
EFFECT: increase of an output of cells of a microorganism.
1 tbl, 8 ex
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|
SU1818340A1 |
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RU2451743C2 |
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RU2822665C2 |
SYNTHETIC NUTRIENT MEDIUM FOR BRUCELLA CULTURING | 1998 |
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RU2148638C1 |
NUTRIENT MEDIUM FOR LEGIONELLA PNEUMOPHILA CULTIVATION | 1989 |
|
RU1614494C |
Authors
Dates
2009-07-20—Published
2007-11-01—Filed