FIELD: biotechnology.
SUBSTANCE: invention is a method of preparing a concentrate of microbial cells for obtaining live tularemia vaccine, which is characterised in that tularemia microbe is grown at a temperature of 37°C for 20±2 hours on the nutrient medium containing, g/l: dry enzymatic hydrolysate of fibrin prepared from waste of whey-vaccine production 50-55, glucose 10, calcium pantothenate 0.05, followed by concentration of the microbial mass by microfiltration of the culture of tularemia microbe through the membranes with the pore size of 0.2 mcm in the mode of tangential liquid flow.
EFFECT: preparing the concentrate of microbial cells of tularemia microbe suitable for obtaining live tularemia vaccine.
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