WAY OF RECEPTION OF ISOTOPE-MARKED C POLYNONSATURATED FAT ACIDS Russian patent published in 2009 - IPC C12P7/64 C12N1/12 

FIELD: medicine; biotechnologies.

SUBSTANCE: way includes cultivation of fresh-water diatoms Synedra Acus in a photobioreactor on the medium containing an isotope ¹³C-containing component. The seaweed selected from a natural source is used for inoculation, or a biomass preliminary enriched by an isotope ¹³C. Cultivation is performed in the conditions excluding access of extraneous sources of carbon from the environment. In quality of isotope-containing reagent bicarbonate NaH¹³CO₃ is used. Further biomass selection, its filtering, homogenising and extracting of the common lipids using an organic dissolvent is performed. Hydrolysis of the common lipids and the subsequent clearing of a target product by chromatography is carriedout. The way allows raising enrichment level with a stable isotope of received polynonsaturated fatty acids. Degree of enrichment by an isotope ¹³C of palmitoleic acid makes 60%, stearin acid - 18%, linolenic acid - 75%, eicosapentanoic acid - 64%.

EFFECT: rising of degree of enrichment by an isotope.

6 cl, 2 dwg, 1 tbl, 2 ex