NUTRIENT MEDIUM FOR MYCOBACTERIAL CULTIVATION IN BACTERIOLOGICAL TUBERCULOSIS DIAGNOSTICS Russian patent published in 2009 - IPC C12N1/20 

Abstract RU 2366700 C1

FIELD: medicine.

SUBSTANCE: nutrient medium for primary-culture mycobacterial cultivation contains a salt base containing: magnesium sulphate - 0.5 g, sodium citrate - 1.0 g, iron alum - 0.05 g, monosubstituted potassium phosphate - 20.0 g, monosubstituted aluminium citrate - 5.0 g, monosubstituted sodium glutamate - 10.0, glycerine - 20 ml, distilled water - to 1000 ml, egg mixture, 2% malachite green and antler cake pantolysate in the ratio as follows, ml: salt base 1000, egg mixture 700-1000, pantolysate 300-1000, 2% malachite green 33. Antler cake pantolysate is prepared by aqueous extraction of antler cake in the ratio cake: water 1:4-1:5 at process temperature 98-99°C under pressure 1.5 atm during 3-4 h.

EFFECT: improved growth qualities and higher information value of the medium.

1 tbl, 4 ex

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RU 2 366 700 C1

Authors

Lunitsyn Vasilij Gerasimovich

Romantseva Julija Nikolaevna

Ashenbrenner Aleksandr Ivanovich

Dates

2009-09-10Published

2008-02-04Filed