METHOD FOR PRODUCTION OF MEDIUM FOR MYCOBACTERIUM TUBERCULOSIS (MBT) CULTURING Russian patent published in 2007 - IPC C12N1/20 

FIELD: biotechnology.

SUBSTANCE: claimed method includes blending of salt base comprising of mineral salts with glycerol, dissolution of salt base and glycerol mixture, providing of malachite green solution, egg mass providing and blending of prepared solutions with egg mass; wherein salt base and glycerol mixture is dissolved in 0.1 % sodium humate solution, and components are used in the next amount: magnesium sulfate (MgSO₄ x 7H₂O) 0.5 g; sodium citrate (C₆H₅O₇Na₃ x 5.5H₂O) 0.1 g; ferrous-aluminum alum (Fe(NH₄)(SO₄)₂ x 12H₂O) 0.05 g; monosubstituted potassium sulfate (KH₂PO₄) 20.0; monosubstituted ammonium citrate (C₆H₁₁O₇N) 5.0; monosubstituted sodium glutaminate (C₃H₈NaO₄ x H₂O) 10.0; glycerol (C₃H₈O₃) 20.0 ml; 0.1 % sodium humate (C₃₉H₆₈O₂₆N₃)_n solution up to 1000.0 ml. Method of present invention make it possible to produce the first generation of MBT cultures by 3 days earlier and to increase biomass by 2.1 times in relates to control.

EFFECT: accelerated method of increased productivity.