FIELD: pharmacology.
SUBSTANCE: invention concerns biotechnology. Method involves: reduction of plasmatic Cohn IV1 fraction; anion exchange chromatography of solution containing alpha-1-antitrypsin on anion exchange gel, preferably DEAE Sepharose® fast flow; concentration of obtained eluate by ultrafiltration; additional chromatography on hydrophobic carrier and processing of fraction containing alpha-1-antitrypsin by material containing immobilised heparin form; further inactivation of viruses covered with lipidic capsule by adding detergents and optional solvent; salting-out of detergents by further salt concentration increase to ≥ 0.5 M; separation of detergents and virus particles by nanofiltration with pore grade of 15-20 nm.
EFFECT: enhanced purity and safety of obtained product.
17 cl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| PREPARATION OF ANTIBODY IgG AND METHOD OF ITS PRODUCTION | 2005 |
|
RU2337109C2 |
| METHOD OF PRODUCING FIBRINOGEN USING STRONG ANION EXCHANGE RESIN AND FIBRINOGEN-CONTAINING PRODUCT | 2011 |
|
RU2603103C2 |
| METHOD OF OBTAINING SAFE IN VIRUS RESPECT BIOLOGICAL FLOWING MEDIA | 2005 |
|
RU2411959C2 |
| METHODS OF VIRUS INACTIVATION USING ENVIRONMENTAL-FRIENDLY DETERGENTS | 2014 |
|
RU2707951C1 |
| METHOD OF PREPARING GAMMA-GLOBULIN SOLUTION DESIGNATED FOR INTRAVENOUS ADMINISTRATION AND PREPARATION PREPARING BY THIS METHOD | 1998 |
|
RU2198668C2 |
| METHOD OF OBTAINING VIRUS-SAFE COMPLETE PROTHROMBIN COMPLEX | 2014 |
|
RU2559576C1 |
| PURIFICATION AND APPLICATION OF FACTOR, CONTRIBUTING TO WOUND HEALING | 2007 |
|
RU2520817C2 |
| METHOD OF PURIFYING COAGULATION FACTOR VIII | 2009 |
|
RU2698392C2 |
| METHOD FOR OBTAINING A LIOPHILIZED PREPARATION OF AN ACTIVATED PROTROMBIN COMPLEX HAVING VIII-SHUNTING ACTIVITY FACTOR | 2017 |
|
RU2648517C1 |
| METHOD FOR PURIFICATION OF BLOOD-COAGULATION FACTOR VIII | 2009 |
|
RU2567811C2 |
