FIELD: pharmacology.
SUBSTANCE: invention represents method of obtaining medication of complete prothrombin complex, which consists in the following: from cryosupernatant, obtained by cryofractioning of freshly frozen plasma, extraction of prothrombin complex, which is further subjected to viral inactivation by solvent/detergent method and chromatographic purification with application of buffer solutions of different ionic power. Content of salts and pH are corrected to the level close to physiological in obtained in such way solution of virus-safe complete prothrombin complex, after which it is concentrated, sterilised by microfiltration and transferred into lyophilised form. Claimed method makes it possible to obtain medication of virus-safe complete prothrombin complex with ratio of blood clotting factors II, VII, IX and X, close to 1:1:1:1.
EFFECT: medication is stable at storage and convenient for application.
2 cl, 1 dwg, 1 tbl, 4 ex
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