FIELD: veterinary biotechnology, immunology. SUBSTANCE: invention relates to preparing the strain of hybrid cultured murine cells producing monoclonal antibodies raised to the light chains of pig immunoglobulins. Strain is obtained by fusion of murine splenocytes of the strain Balb/c immunized with the surface pig IgA with myeloma Sp 2/O cells. Antibodies relate to isotype IgG1 and stimulate precipitating activity of monoclonal antibodies to pig IgG. Invention can be used for development of diagnostic reagents for detection of immunoglobulins in pig biological fluids. EFFECT: strain indicated above, enhanced effectiveness of detection. 2 tbl
Authors
Dates
1997-10-20—Published
1995-03-31—Filed