FIELD: medicine.
SUBSTANCE: invention concerns biotechnology. The method involves splenectomy of an animal, infection with blood parasites, exsanguinations at invasion peak, blood conservation and use for making antigens. Thus before infection, autoblood is accumulated by fivefold draw and transfusion of autoblood in amount 150, 300, 500, 700 and 1000 ml respectively with isotonic solution every 3-4 days, then an animal is infected with Babesia canis. At parasitemia peak, when 30-50% of erythrocytes involved, the blood is taken twice every 3-4 days in amount 800-900 ml, and then the prepared autoblood is injected. Then at the third parasitemia peak, the animal is exsanguinated completely, and the blood prepared at three stages is used for making antigen.
EFFECT: method allows producing greater blood volume from one animal.
1 tbl
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Authors
Dates
2010-01-27—Published
2008-07-03—Filed