METHOD FOR PREPARING ANTIGEN FOR SERUM DIAGNOSIS OF SMALL CATTLE ANAPLASMOSIS Russian patent published in 2014 - IPC A61K39/00 G01N33/53 

FIELD: medicine.

SUBSTANCE: invention refers to veterinary protozoology and concerns a method for preparing an antibody for serum diagnosis of small cattle anaplasmosis. The presented method involves introducing an infective dose of 14·10¹⁰ or 16·10⁷ anaplasma/animal on the 15^th or 22^nd-24^th day respectively into a splenectomised animal, carrying out exsanguination, centrifuging blood for 2-3 times at 6000 rpm for an hour with physiological solution at pH 6.8-7.2, preparing the anaplasma residue with blood corpuscles, washing in physiological solution for 2-3 times, performing erythrocytoschisis by 2-3 cycles of freezing and thawing, concentrating for 2-3 times by centrifugation in the same environment and recovering the antigen with titre 1:32-1:64.

EFFECT: method enables preparing the sensitive, high-active and specific anaplasma antigen and can find application in veterinary laboratories specialised in diagnosis of infectious and protozoal diseases.

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