FIELD: medicine.
SUBSTANCE: invention concerns method for simultaneous detection of clinically important steroids in human biological fluid used for screening of gynaecologic and oncologic diseases caused by disturbed synthesis and metabolism of steroid hormones. The method involves extraction of steroid conjugates, solid content recovery and cleavage with simultaneous protection of functional groups. Steroid conjugates are fluid-fluid extracted in ethyl acetate medium. Steroid conjugates are cleaved by combined acid hydrolysis at temperature 80°C through reaction of benzene, steroid conjugates and hydrochloric acid diluted to 1:2, with a metal ball added.
EFFECT: higher effectiveness of the method.
3 cl, 1 ex, 1 tbl, 2 dwg
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