METHOD FOR MAKING ANTIBODY TEST SYSTEM FOR LATEX FIXATION TEST Russian patent published in 2010 - IPC G01N33/531 

FIELD: medicine.

SUBSTANCE: invention concerns method for making an antibody test system based on polymer microspheres - bioligand carriers (protein A, specific immunoglobulin G) for detecting antigens: Yersinia enterocolitica serotype 03, Leptospira canicola, Salmonella pulorum, virus of avian infectious bronchitis (AIB) strain H120. Method for making the antibody test system consists in mixing equal volumes of physiologic saline containing protein A (strain Staphylococcus aureus) and polymer microspheres of 0.5% polymer suspension. The mixture is placed in a thermostat at +36-38°C. The polymer suspension is washed, and the deposit is mixed with physiologic saline containing specific IgG to one of antigens. The prepared mixture is kept in the thermostat at +36-38°C. The polymer suspension is washed, while the deposit is mixed with physiologic saline containing albumin.

EFFECT: application of the method allows making the high-sensitive test system for latex fixation test in detecting antigens of Yersinia enterocolitica, Leptospira canicola, Salmonella pulorum, and AIB virus.

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